The Department of Transfusion Medicine at KIMSHEALTH Hospital Trivandrum is a NABH-accredited and licensed Blood Centre, operating 24/7 to provide essential support to patients requiring blood transfusions. Our department is equipped with robust processes and protocols to meet the evolving needs of both patients and clinicians.

To ensure safe and efficient blood collection, component separation, and storage, we utilize state-of-the-art technologies and infrastructure. We are proud to be one of the few centres in the country with NABH accreditation which we are continuously maintaining since 2012.

To encourage and promote voluntary blood donations, we offer complimentary valet parking for all donors visiting our centre. Additionally, donors who contribute at least three times a year receive a complimentary annual health check-up.

Our commitment to patient safety is clearly reflected in our clinical outcomes. The annual incidence rates for allergic reactions and Febrile Non-Hemolytic Transfusion Reactions (FNHTR) are notably low at 0.17% and 0.14%, respectively. These figures are below several national and international benchmarks, underscoring our dedication to excellence in transfusion medicine.

Our Highlights

• 100% blood component therapy ensured for all patients
• 100% NAT (Nucleic Acid Testing) screened blood components for enhanced safety
• Universal leukodepletion -All PRBC (Packed Red Blood Cell) units are leukodepleted
• Zero incidence of ABO mismatch transfusions
• Active participant in the National Hemovigilance Programme of India
• Availability of therapeutic and donor apheresis including CAR-T cell apheresis
• Peripheral blood stem cell collection and cryopreservation facilities are available
• Nucleic acid Technology for Transfusion transmitted infection screening
• Advanced regenerative therapies including PRP (Platelet-Rich Plasma) and PRF (Platelet-Rich Fibrin)
• Flowcytometry based HLA crossmatching facilities available to support both cadaveric and live solid organ transplants
• Other Comprehensive flow cytometry services, including CD34 enumeration, CD4, CD19, CD20 counts, and diagnostic panels for acute leukaemia and chronic lymphoproliferative diseases

Blood Donor Services

Comprehensive care and assessment for voluntary blood donors and apheresis donors.

• Blood Donor Counselling and Pre-medical Examination
• Blood Collection with all safety precautions
• Donor apheresis for Single Donor Platelets and single donor plasma
• Granulocyte Collection
• Autologous stem cell collection

Component Preparation and Processing

We process whole blood into high-quality blood components suitable for transfusion. Additionally, we provide modified blood components tailored to the specific demands from the clinical area.

• Packed Red Blood Cells (PRBC)
• Fresh Frozen Plasma (FFP)
• Platelet concentrates
• Cryoprecipitate
• Leukodepleted Cellular Blood Components
• Irradiated Blood Components
• Washed Cellular Components
• Pooled blood components

Basic and Advanced Immunohematology Testing

Ensuring transfusion safety through,

• Blood Grouping and Rh Typing
• Crossmatching /Compatibility testing
• Antibody Screening
• Antigen Typing
• Antibody Identification
• Red cell Phenotyping for antigens
• Direct Antiglobulin Test (DAT)
• Monospecific DAT testing
• Adsorption and elution techniques
• Saliva testing

Infectious Disease Screening for Donor Units

Our Blood Centre employs a dual screening methodology to ensure maximum safety from transfusion-transmissible infections.

• TTI Screening using Enhanced Chemiluminescence (ECI) Technology
• NAT Screening for Virology Markers – HIV, HBV, HCV

Advanced Cellular Therapies

Specialised cellular collection and preservation services for haematology and oncology needs.

• Peripheral Stem Cell Collection
• Cryopreservation
• CAR-T Cell Apheresis

Therapeutic Procedures

Innovative transfusion-based therapies for treating complex medical conditions.

• Therapeutic Plasma Exchange
• Leukocytapheresis

Regenerative Therapies

Biological therapies aimed at tissue repair and healing.

• Platelet-rich plasma (PRP)
• Platelet-rich fibrin (PRF)

Diagnostic & Treatment Support

• Flow cytometry technology
• ROTEM (Rotational Thromboelastometry)

FLOWCYTOMETRY LABORATORY

OVERVIEW

Flowcytometry is a cutting edge technology that analyses the physical and chemical properties of cells or particles in a fluid as they pass through a laser beam

Our state of the art flowcytometry lab provides advanced diagnostic solutions, enables accurate detection of blood disorders, immune deficiency and cancer markers, all with precision and speed.

Why choose KIMSHEALTH Flowcytometry Lab

• Advanced technology
• Expert team
• Accurate results
• Quick turn around time
• Comprehensive panels

Applications in patient care:

• Oncology
• Transplantation
• Infectious diseases
• Immunodeficiency diseases

SERVICES OFFERED:

• HLA Flow crossmatch
• Stem cell CD34 enumeration
• Acute leukemia panel
• Chronic lymphoproliferative disorder / CLPD Panel
• CD 4/8 counts
• CD 19 and CD 20 counts
• Lymphocyte subset analysis (TBNK)

Test Description

HLA Flowcrossmatch

HLA flow crossmatch (FCXM) is a test used to assess compatibility between a donor and recepient.It evaluates whether the recipeint has antibodies against donor human leukocyte antigens, which could lead to organ rejection post transplant. FCXM has much more sentisitvity than CDC crossmatch

Stem Cell CD34 Enumeration

CD34 stem cell enumeration by flowcytometry is used to quantify CD34+ hematopoietic stem cells in the blood sample. It helps to evaluate,

• To evaluate the suitability of stem cell donors or grafts for transplantation.
• To quantify CD34 cells collected during peripheral blood stem cell mobilization or harvest to ensure an adequate dose for transplantation.

Acute Leukemia Panel

Acute leukemia panel is a diagnostic test used to evaluate and classify leukemia specifically acute leukemia. This panel uses flowcytometry to identify and quantify abnormal cells in bone marrow, blood or other tissue by analyzing the expression of specific surface and intracellular markers. It can be used:

• To identify leukemia subtypes based on immunophenotyping
• To classify leukemia and differentiate it from lymphoma
• To assess markers associated with prognosis and guide treatment decisions
• To identify targeted markers for therapy for monoclonal antibody treatments

Chronic Lymphoproliferative Disorder / CLPD Panel

The chronic lymphoproliferative disorder (CLPD) panel or Lymphoma panel by flowcytometry is a diagnostic test designed to evaluate lymphoid malignancies. It helps in evaluating and sub categorize non-Hodgkin’s lymphoma. It can be used

• To confirm or rule out chronic lymphoproliferative disorders
• To distinguish between reactive lymphocytosis and malignant lymphoproliferation
• To identify cell surface markers that indicate the aggressiveness of the disease.

CD4/8 Counts

The CD4/8 count is performed to quantify specific types of T lymphocytes (T helper and cytotoxic) in the immune system. These counts are commonly used in immunology and infectious disease management, particularly monitoring conditions like AIDS/HIV, autoimmune diseases etc.

CD 19 and CD 20 Counts

CD19/CD 20 count flow cytometry is a test that measures the number of CD19+ B cells in the body to help diagnose and monitor conditions that affect the immune system. It is used to evaluate treatment response to anti CD-19 /Anti CD 20 monoclonal antibodies

Lymphocyte Subset Analysis (TBNK)

This is a basic immunodeficiency screening panel and is used to monitor and evaluate the immune system

TECHNOLOGY AND INFRASTRUCTRE

BD FACS LYRIC

High perfomance instrument designed by BD for advanced flow cytometry applications with 3 laser, 12 color detection capacity and also advanced sensitivity and resolution capacity .

SOFTWARE USED FOR ACQUISITION OF EVENTS AND DATA ANALYSIS

• BD FACS SUITE
• BD FACS CLINICAL
• BD FACS DIVA

EXPERTISE AND TEAMS

1. Dr. Sanooja Pinki S, MD, DNB, Head and Consultant in Transfusion Medicine, Certiifed in Flowcytometry
2. Dr. Neetu S. Dathan, MD, Fellowship Oncopath) Consultant, Department of Pathology
3. Dr. Deepthi B, MD, DNB, Fellowship (Oncopath) Consultant, Department of Pathology
4. Dr. Padmini R, MD, DNB, Consultant, Department of Pathology

TAT AND REPORTING

Normal TAT for all the above tests is 48 hrs after the sample is received at KIMS Health except for CD34 enumeration (TAT excludes Sundays)

Empower your research and clinical diagnostics with cutting edge flowcytometry technology Contact us to discover how our solutions can accelerate your success

KIMSHEALTH -FLOWCYTOMETRY lABORATORY Our contact details: 0471-294-2227 / 0471-294-1216 E mail: flowlab.tvm@kimsglobal.com